A differential medium support the growth of any microbe but distinguishes them based on how they metabolize or change the medium. DNase agar is a differential medium that tests the ability of an organism to produce an exoenzyme, called deoxyribonuclease (DNase) that hydrolyzes DNA. The streptococci are classified by their hemolytic activity. Differential media contain compounds that allow groups of microorganisms to be visually distinguished by the appearance of the colony or the surrounding media, usually on the basis of some biochemical difference between the two groups. It is the selective and differential medium for coliforms. For example in case of Mannitol salt agar, it contains mannitol and phenol red as pH indicator which support the … The difference between selective and differential media is mainly due to the following factors: Intended use of the media: The purpose of both selective and differential media differs.A selective media only permits the development of particular organisms, while a differential media discerns one species of the organism from the other growing on the similar substrate. Subject. Many are significant pathogens, such a coli, Salmonella, Shigella, but many compose our normal flora and inhabit soil and water. Time-lapse clips of selective and selective/differential media. EMB contains dyes that are toxic to Gram-positive bacteria. E salts contain only MgSO4, K2HPO4, Na2NH4PO4, and citric acid, and therefore, in order to grow, an organism must be prototrophic (capable of synthesizing, opposite is auxotrophic) for all necessary molecules such as amino acids, Microbiology. Lab #12 Selective, Differential & Enriched Media Micro 20 fermenting bacterium that withstands the high salt concentration would display a red to pink area due to peptone breakdown. ENRICHMENT MEDIA |Basic principle is to control the nutrients and culture conditions in such a way that it suits mainly to a specific species ytemperature, air supply, light, pH |When we assume low amount of potential pathogens being present in the specimen, they have to be enriched first, to multiply up the low Browse selective and differential media types in broth or agar for Escherichia coli (E. coli) and coliforms. Differential media is like blood agar, which is an organism in itself, but seeks to destroy another one (hemoglobin). ... › Selective and differential media quizlet show more less. Selective/Differential Media. Bingo. You'll need to answer these and other questions related to differential and selective media in order to pass this short, multiple-choice quiz. EMB is the selective and differential medium for coliforms.It is a blend of two stains, eosin and methylene blue in the ratio of 6:1. Microbial Metabolism & Specialized Bacterial Growth Media Homework Assignment Name: _____ The purpose of this exercise is to help students improve their understanding of selective and differential bacterial growth media, and how the media allows for the identification bacteria based on metabolism. Differential Media These two types of media can – often in a single step – give a preliminary ID for an infectious organism Selective! These media are non selective and they contain general nutrients which required for the growth of wide range of microorganisms. Add to Cart. Eosin methylene blue (EMB, also known as "Levine's formulation") is a selective stain for Gram-negative bacteria. Culture. Matching. A blood differential test, also known as a white blood cell count differential, measures the number of different types of white blood cells in your blood. Created. Study Flashcards On Microbiology: Lab Exercise 10-Selective, Enriched and Differential Media at Cram.com. Helpfulness: +1. Quizlet.com Selective and differential media is extremely useful when examining mixed bacterial cultures since it provides: a simple way to "screen out" certain bacterial species some biochemical information on the organisms present in the culture (presumptive identification) Media can be Selective and Differential Description. BAP is rich in nutrients and contains sheep blood. It is not selective, and will grow many different types of microbes. Selective media allow certain types of organisms to grow, and inhibit the growth of other organisms. Total Cards. So, media could be both selective as well as differential. Principle: Differential growth suppression Selective medium is designed to suppress the growth of some microorganisms while allowing the growth of others. Undergraduate 2. Embed this set cancel. Synonym. Selective media allow certain types of organisms to grow, and inhibit the growth of other organisms. COPY. 3.8 - Selective vs. No Chapter. The MSA will select for organisms such as Staphylococcus species which can live in areas of high salt concentration (plate on the left in the picture below). Selective and differential media are used to isolate or identify particular organisms. coagulase test quizlet, Principle of the Test Traditionally, differentiation between coagulase-positive and coagulase-negative staphylococci has been performed either with the tube coagulase test that detects extracellular staphylocoagulase or the slide coagulase test that detects the clumping factor (bound coagulase) present on the bacterial cell surface. Examples of selective media include: Eosin methylene blue contains dyes that are toxic for Gram-positive bacteria. Differential and selective media are commonly used in microbiology laboratories. Quickly memorize the terms, phrases and much more. The selectivity is accomplished in several ways. Print Print. Enjoy the videos and music you love, upload original content, and share it all with friends, family, and the world on YouTube. Selective media enables one type of bacteria to grow, while differential media allows bacteria to show different reactions (i.e. Application. Selective media is designated for the growth of one type of organism. Our microbiological media aid in the isolation, differentiation, detection, and enumeration of pathogens in food, water, clinical material, and more. Includes 6.5 % Salt, M-staph, M.S.A, Bile Esculin, EMB, MacConkey and Hektoen Enteric Agar. One example of differential medium is blood agar. Set Details Share. So, it's a confirmatory media, rather than a selective media. Selective medium is agar-based (solid) medium so that individual colonies may be isolated. From history and the arts to travel and fashion, we take a broad look at culture in the context of sustainability. Selective media microbiology quizlet keyword after analyzing the system lists the list of keywords related and the list of websites with related content, in addition you can see which keywords most interested customers on the this website. Mannitol Salt Agar (MSA) This type of medium is both selective and differential. Nutrient media: These media are also called general purpose media. The MacConkey’s and Mannitol Salt described above are both selective and differential bacterial growth media. Selective and Differential Media If a bacterial growth medium is selective , that means that it grows only certain types of microbes while inhibiting the growth of others. Selective and differential media are used to isolate or identify particular organisms. Cram.com makes it … colony color) Fig. While differential media are used to differentiate closely related organisms or groups of organisms. Quiz. 10/11/2012. Blood agar medium is, however, differential. Bacterial Identification Lab Handout Quizlet ESO, European Organisation for Astronomical Research in the Southern Hemisphere By continuing to use this website, you are giving consent to our use of cookies. Examples of selective media … Page to share: COPY. Selective and Differential Media EG medium is a defined minimal medium consisting of 1x E salts + 0.5% glucose. Blood Agar (BAP) is another type of differential medium. Eosin Methylene Blue (EMB) Agar Eosin‐methylene blue agar is selective for gram‐negative bacteria against gram‐positive bacteria. Some media are both differential and selective. a. Many gra negative bacilli that inhabit our large intestine are called enterics. ; MacConkey agar is for Gram-negative bacteria. Microbiology an Introduction. Selective and differential media; EMB and Tsl agar bacill inhabit a wide range of environments. 31. Quiz & Worksheet Goals. This is in contrast to Streptococcus species, whose growth is selected against by this high salt agar (plate on the right in the picture below). Selective medium. Differential media are those media which are used to distinguish closely related microorganisms based on their morphology on different agar media. DNase agar does NOT contain selective agents. Some specialized media are both selective and differential, others are either or. Part 2: Different types of selective and differential media, and identification of microorganisms depending on growth or colour change. ; Hektoen enteric agar is selective for Gram-negative bacteria. Types of Media - Selective and Differential Media. YM (yeast extract, malt extract agar) has a low pH, deterring bacterial growth. Differential media typically display some type color change in the presence of certain bacteria. updated 5 years ago by mmiyakooo. Level. 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